custom built tirf microscope (Nikon)
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Nikon
custom built tirf microscope
Custom Built Tirf Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom+built+tirf+microscope/ECLIPSE+Ti2/pm41722048-415-6-15
Average 99 stars, based on 11616 article reviews
Custom Built Tirf Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 11616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom+built+tirf+microscope/ECLIPSE+Ti2/pm41722048-415-6-15
Average 99 stars, based on 11616 article reviews
custom built tirf microscope - by Bioz Stars,
2026-09
99/100 stars
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Microscopy:Article Title: MRAP2 potentiates GPCR signaling by conserved mechanisms that are disrupted by obesity-associated genetic variants Article Snippet: .. HILO images were acquired on a Article Title: A calcium-sensing receptor dileucine motif directs internalization to spatially distinct endosomal signaling pathways Article Snippet: For studies with SNAP-CaSR, SNAP-Surface Alexa Fluor 647 (NEB) was diluted 1:1000 in FluoroBrite complete media and applied to cells for 20-minutes, before washing and imaging. .. HILO images were acquired on a Article Title: Revealing the tissue-level complexity of endogenous glucagon-like peptide-1 receptor expression and signaling. Article Snippet: The next day cells were incubated with SNAP-Surface549 (New England Biolabs) in complete culture medium for 20min at 37 °C and washed 3 × 5min using HEPES-bicarbonate buffer, leading to labeling of ~80% of surface GLP1R required for single-molecule analysis. .. GLP1RSNAP/SNAP islet cells were then imaged in HEPES-bicarbonate buffer supplemented with 16.7mM D-glucose using a Article Title: Regulation of Dynein Motility and Force Generation by Lissencephaly-1 Article Snippet: .. Single-molecule imaging was performed using a Article Title: Investigation of Inactive-State κ Opioid Receptor Homodimerization via Single-Molecule Microscopy Using New Antagonistic Fluorescent Probes. Article Snippet: .. After washing the coverslip with 1 mL of imaging buffer (137 mM NaCl, 5.4 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 10 mM HEPES, pH 7.3), the cells were imaged in 300 μL imaging buffer at 20 oC, using a Article Title: MRAP2 potentiates GPCR signaling by conserved mechanisms that are disrupted by obesity-associated genetic variants. Article Snippet: .. HILO images were acquired on a Article Title: Revealing the tissue-level complexity of endogenous glucagon-like peptide-1 receptor expression and signaling Article Snippet: The next day cells were incubated with SNAP-Surface549 (New England Biolabs) in complete culture medium for 20 min at 37 °C and washed 3 × 5 min using HEPES-bicarbonate buffer, leading to labeling of ~80% of surface GLP1R required for single-molecule analysis. .. GLP1R SNAP/SNAP islet cells were then imaged in HEPES-bicarbonate buffer supplemented with 16.7 mM D-glucose using a Imaging:Article Title: Regulation of Dynein Motility and Force Generation by Lissencephaly-1 Article Snippet: .. Single-molecule imaging was performed using a Article Title: Investigation of Inactive-State κ Opioid Receptor Homodimerization via Single-Molecule Microscopy Using New Antagonistic Fluorescent Probes. Article Snippet: .. After washing the coverslip with 1 mL of imaging buffer (137 mM NaCl, 5.4 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 10 mM HEPES, pH 7.3), the cells were imaged in 300 μL imaging buffer at 20 oC, using a Inverted Microscopy:Article Title: Regulation of Dynein Motility and Force Generation by Lissencephaly-1 Article Snippet: .. Single-molecule imaging was performed using a |
![( A ) Illustration of the single molecule imaging assay, which combines microfluidics, surface immobilization and <t>TIRF</t> microscopy. ( B ) Typical field-of-view showing individual flow-aligned DNA strands (green) with MpARF2 nanoclusters (magenta) bound to the DNA templates. [MpARF2] = 1 nM. Scale bar: 5 µm. Inset: filtered Region Of Interest (ROI). ( C ) Kymograph of a nanocluster binding to DNA, illustrating the high temporal stability of a nanocluster once bound, with virtually no lateral movement. ( D ) Binding isotherm of nanoclusters, determined from the area fraction of protein covering the DNA, ϕ . Inset: binary-filtered ROIs used in coverage analysis. ( E ) Spatial distribution of binding events along individual DNA strands (solid) compared to the predicted IR7 positions (dashed). Inset: enrichment of protein on nonspecific (green) and IR7-containing (orange) DNA templates, measured as the ratio of protein intensity on DNA compared to the surrounding background. *** P <0.001; Welch’s t-test.](https://bio-rxiv-images-cdn.bioz.com/dois_ending_with_32/10__1101_slash_2025__11__05__686732/10__1101_slash_2025__11__05__686732___F4.large.jpg)